Journal article

Chronic ocular hypertension induced by circumlimbal suture in rats

HH Liu, BV Bui, CTO Nguyen, JM Kezic, AJ Vingrys, Z He

Investigative Ophthalmology and Visual Science | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | Published : 2015

Abstract

PURPOSE. To induce chronic intraocular pressure (IOP) elevation in rat eyes by circumlimbal suture.METHODS. Anesthetized (isoflurane) Long-Evans rats underwent unilateral circumlimbal suture implantation while the fellow eyes served as untreated controls (n ¼ 15). A sham group (n ¼ 8) received the same procedure except that the suture was loosely tied. Intraocular pressure, electroretinography (ERG), and optical coherence tomography (OCT) were monitored for 15 weeks, after which retinal histology and immunofluorescence staining for glial fibrillary acidic protein (GFAP) and ionized calcium binding adapter molecule-1 (Iba-1) were undertaken.RESULTS. Both IOP and ERG remained unaltered in the ..

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